顯微調(diào)制熒光儀Microscopy-PAM

簡(jiǎn)要描述：利用PAM主機(jī)連接顯微鏡，測(cè)量1個(gè)或多個(gè)細(xì)胞的光合作用，不需分離培養(yǎng)即可測(cè)量自然水樣中特定藻細(xì)胞的光合作用。

更新日期：2025-06-18
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Microscopy-PAM **臺(tái)可以測(cè)量單細(xì)胞光合作用的熒光儀
Schreiber教授因發(fā)明PAM系列調(diào)制葉綠素?zé)晒鈨x而獲得首屆光合作用協(xié)會(huì)（ISPR）創(chuàng)新獎(jiǎng)

以通用控制單元PAM-Control為主機(jī)，連接顯微鏡，結(jié)合熒光配件，可以測(cè)量單個(gè)細(xì)胞或數(shù)個(gè)細(xì)胞的光合作用。

研究對(duì)象可以為浮游植物或葉綠體

研究自然水樣中的浮游植物生理活性時(shí)，一般步驟是：首先分離純化（多為顯微鏡下用毛細(xì)管挑藻），獲得純種細(xì)胞后擴(kuò)大培養(yǎng)，然后研究其生理活性。這個(gè)過(guò)程至少需要2－3個(gè)月。而且有些在自然條件下經(jīng)常發(fā)生水化/赤潮的微藻，分離后總是無(wú)法成功養(yǎng)活（特別是海洋甲藻）。

現(xiàn)在，只需取水樣回來(lái)，在顯微鏡視野中找出您感興趣的1個(gè)或幾個(gè)細(xì)胞，就可檢測(cè)它（們）的光合作用活性，省去了分離培養(yǎng)的步驟，大大節(jié)省了時(shí)間。對(duì)許多至今仍無(wú)法分離培養(yǎng)成功的藻類，也可研究其生理活性。

部分文獻(xiàn)

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